FastQCFastQC Report
Sat 18 Mar 2023
SRR4895600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987094
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72500.36485440547855313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT45500.22897759240378157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTTA33960.1709028360007126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG31910.1605862631561466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA29780.14986709234691464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC29530.14860897370733342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25150.1265667351418705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24860.1251073175199563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA23700.11926964703229943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC23620.11886704906763344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGACCAGGTGCGGTGGCTC23220.1168540592443035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT415350.0135.793416
GGTATCA417850.0135.153268
AAGCAGT419450.0134.603911
TGGTATC420400.0134.282097
AGTGGTA420700.0134.169225
GTATCAA423000.0133.490759
CAGTGGT428200.0131.872994
AGCAGTG429250.0131.533632
GCAGTGG450200.0125.348763
CAACGCA415150.027.96257210-14
TCAACGC416800.027.80696310-14
ATCAACG416600.027.75809710-14
GTACATG418200.027.62435220-24
AACGCAG419150.027.5720510-14
TACATGG419550.027.56635520-24
CGCAGAG419850.027.52950715-19
TACGCAC7150.027.3887550-54
AGAGTAC421450.027.38741315-19
AGTACAT421900.027.36502520-24
CAGAGTA426050.027.19648615-19
Produced by FastQC (version 0.11.5)