FastQCFastQC Report
Sat 18 Mar 2023
SRR4895599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814813
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT495612.7309149758129347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCA1823550.0143.309042
CAACCAG618200.0143.242953
AACAACC1826900.0143.046261
CAACCAT656800.0142.991473
AACCATG267000.0141.734824
AACCAGT215750.0141.597214
CAACCAA555450.0141.407523
ACCATAG68850.0140.967325
AACCATA196050.0140.290744
AACCAGA189400.0140.274554
AACCAGG222700.0139.926354
ACCATTG75150.0139.880235
ACCATGA62150.0139.366045
ACCAGTG71000.0139.335225
ACCAATG72850.0138.95955
ACCAGAG65950.0138.65055
AACCAAG192000.0138.04
ACCAGAT66800.0137.748495
CCATATG25700.0137.276266
AACCAAT203250.0137.269384
Produced by FastQC (version 0.11.5)