FastQCFastQC Report
Sat 18 Mar 2023
SRR4895599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814813
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG453482.498769845708621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC95230.524737259431137No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73860.4069840804534682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA61540.33909829828197174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG51840.28564926524110196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT44220.24366146815126408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA39990.2203532815777714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC38140.21015939383286325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36730.20238999830836565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA33590.185087940189981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA29930.1649205730838384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27640.15230219311851964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT22140.1219960403633873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20480.11284909244092918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGACGCGGTGGCTCA20190.11125113165929491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT565900.0100.972811
AGCAGTG582700.098.129392
CAGTGGT590000.096.878644
GGTATCA591750.096.652968
TGGTATC591800.096.596137
GTGGTAT592600.096.404986
AGTGGTA595000.096.040325
GTATCAA599100.095.491229
GCAGTGG602500.094.856763
CATGGGG248850.025.89981325-29
TAAGCAG11550.025.67551
CGATAGG6800.024.35191535-39
TACGCAC5500.023.82445150-54
ATGGGGG146150.023.42918825-29
CGACCTA5500.023.30083845-49
TAACACG22600.022.556046120-124
ATGGGGT27350.022.53360425-29
CAGTTCG8600.022.436264100-104
GCGCGGT40000.021.95907635-39
CTCACGC53400.021.62605745-49
Produced by FastQC (version 0.11.5)