FastQCFastQC Report
Sat 18 Mar 2023
SRR4895598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813422
Sequences flagged as poor quality0
Sequence length150
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG2816850.0143.823272
AGTACAA2822600.0143.530271
TACAAGT980000.0143.140033
TACAAGG965450.0142.7023
TACAAGA883750.0142.679813
ACAAGTG335250.0141.980854
ACAAGTA310300.0141.540074
ACAAGAT311900.0141.021764
CAAGTAT109600.0140.18945
CAAGGAT106150.0139.997765
ACAAGGT341700.0139.975054
ACAAGTT346900.0139.786324
ACAAGAG312550.0139.59974
ACAAGGA297200.0139.469364
CAAGAGT111350.0139.214725
CAAGTAG105300.0138.871445
CAAGGTA114850.0138.48295
CAAGATA103650.0138.3735
CAAGTGT120450.0138.261175
CAAGATG109100.0137.994145
Produced by FastQC (version 0.11.5)