FastQCFastQC Report
Sat 18 Mar 2023
SRR4895598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813422
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118330.4205910098094065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA64970.23092874087143697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC55050.19566918862509783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG44280.15738840458345743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGGCACAGTAGCTC43000.1528387849387685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT42970.1527321532283461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA34180.1214890620745839No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG32730.11633519607083473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA32070.113989298441542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA517550.0133.254338
GTGGTAT519400.0132.696556
TGGTATC520800.0132.339837
AGTGGTA524700.0131.424775
AAGCAGT525300.0131.209641
GTATCAA526500.0131.098539
CAGTGGT533550.0129.247134
AGCAGTG536950.0128.40192
GCAGTGG558350.0123.416153
CAACGCA514700.027.49504710-14
TCAACGC517550.027.318610-14
ATCAACG518800.027.20559310-14
GTACATG518900.027.06485720-24
CGCAGAG522500.026.97179615-19
AGAGTAC520900.026.9385415-19
AGTACAT523600.026.83566320-24
CAGAGTA526000.026.80327415-19
TACATGG524700.026.80136120-24
AACGCAG526050.026.78685210-14
GAGTACA517800.026.70521520-24
Produced by FastQC (version 0.11.5)