FastQCFastQC Report
Sat 18 Mar 2023
SRR4895597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530158
Sequences flagged as poor quality0
Sequence length150
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA1531250.0143.89611
ACGTGAT1531350.0143.886692
CGTGATG666650.0143.772743
CGTGATA386100.0143.738483
CGTGATT478450.0143.367513
GTGATGA178000.0141.734384
GTGATGT324100.0140.267384
GTGATTA139500.0139.35444
GTGATGG179000.0138.730294
GTGATAT190200.0138.359194
GTGATAA122650.0138.18794
GTGATAG89050.0136.561054
TGATGAG73600.0135.293055
TGATGTG139750.0134.674355
GTGATTG116550.0134.60964
TGATATG79350.0133.564795
GATGTGA30600.0130.823126
TGATGTA78500.0130.516785
GATGTGG64400.0129.800846
TGATTAG58400.0129.574945
Produced by FastQC (version 0.11.5)