FastQCFastQC Report
Sat 18 Mar 2023
SRR4895597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530158
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC115930.7576341789540688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG67210.4392356867722157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49080.3207511903999456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA42500.27774909519147695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC33060.21605611969482888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29790.19468577754715527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG29110.1902417920240916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT27730.1812231155214037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA24080.15736936969907683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC21890.14305712220568073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACAATGGCTCACTCCT21750.14214218400975587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT20920.136717907562487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGTTCA20250.13233927476770374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT19370.12658823467903316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCAGGCACTGTGGCTCA19030.12436624191750133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA18280.11946478729647526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA18200.11894196547023249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC16220.10600212527072368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT16100.1052178925313595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCACAATGGCTCACTCCTG15720.10273448885670629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAT15700.1026037834001456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT401800.0136.136761
GGTATCA407250.0134.217548
GTGGTAT407350.0134.113896
AGCAGTG408750.0133.694142
TGGTATC410200.0133.234747
GTATCAA413200.0132.424229
CAGTGGT414950.0131.731234
AGTGGTA415550.0131.536745
GCAGTGG427750.0127.7219853
TAAGCAG8250.038.5735861
CAACGCA403700.027.86783810-14
TACATGG403950.027.64383720-24
ATCAACG406050.027.62144310-14
TCAACGC407250.027.57894710-14
CGCAGAG406750.027.50310515-19
GTACATG406800.027.45370720-24
CAGAGTA408950.027.41148615-19
GCAGAGT409500.027.35005215-19
AGTACAT408500.027.32535720-24
AGAGTAC408150.027.3135115-19
Produced by FastQC (version 0.11.5)