FastQCFastQC Report
Sat 18 Mar 2023
SRR4895596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413912
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG67440.27938052422789234No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30470.12622663957923902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGC2416500.0143.844351
GTAGCCG843850.0143.700643
TGTAGCC2419600.0143.660052
TAGCCGA259900.0143.583724
GTAGCCT793100.0143.481833
GTAGCCA772850.0143.412353
TAGCCGT297800.0143.322314
TAGCCTA255300.0143.181434
AGCCGTA99450.0142.91335
TAGCCGG286350.0142.767234
TAGCCTG270200.0142.640294
TAGCCAG258150.0142.493184
AGCCGAT91950.0142.433235
TAGCCAT271200.0142.353274
AGCCGAA85950.0141.73755
TAGCCAA247950.0141.531054
AGCCGGA91350.0141.161855
TAGCCTT272900.0141.070744
AGCCTGA86050.0140.903415
AGCCTAT87900.0140.886665
Produced by FastQC (version 0.11.5)