FastQCFastQC Report
Sat 18 Mar 2023
SRR4895596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413912
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG136490.5654307199268241No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89830.37213452685930554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC61320.25402748733176683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA54560.22602315245957597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC54280.22486320959504735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT49340.20439850334229248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC42050.17419856233367248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG40290.1669074928994926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG36100.1495497764624394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA35350.14644278664673774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA26410.10940746804357407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTGCAGTGGCTTA24400.10108073533749366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT546750.0126.23321
GTGGTAT549550.0125.427116
GGTATCA550850.0125.327148
AGTGGTA552650.0124.7626345
TGGTATC553500.0124.623067
GTATCAA556200.0124.212259
AGCAGTG563250.0122.498982
CAGTGGT563750.0122.3009344
GCAGTGG576900.0119.56313
CGATAGG15400.026.55417435-39
CATGGGG322950.025.9357625-29
TACGCAC5800.025.81906750-54
CAACGCA549100.025.7536910-14
TCAACGC551050.025.6573310-14
ATCAACG550400.025.6536210-14
GTACATG548600.025.63280320-24
TACATGG549750.025.56346920-24
AGTACAT551300.025.49159620-24
AGAGTAC552150.025.45235315-19
CGCAGAG554600.025.40222515-19
Produced by FastQC (version 0.11.5)