FastQCFastQC Report
Sat 18 Mar 2023
SRR4895595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113986
Sequences flagged as poor quality0
Sequence length150
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGCTAGATATATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.14738652115172038No Hit
ACAAGCTAGAGGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.11404909374835505No Hit
ACAAGCTATTTTAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.11317179302721386No Hit
ACAAGCTATGTGTGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.11229449230607266No Hit
ACAAGCTAGTGAAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.10878528942150789No Hit
ACAAGCTATGTGGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10527608653694313No Hit
ACAAGCTATTGATTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.10001228221009598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGA100.0072307424144.08
GCTATCG100.0072307424144.05
GCTATAT3550.0144.05
CTAAGAG1300.0144.06
GCTAGTG5900.0144.05
GCTAGTA4450.0144.05
GCTAGGA4550.0144.05
TAGGATG400.0144.07
AGCTATG13950.0144.04
GGATTAG100.0072307424144.09
GCTAAGC100.0072307424144.05
CTAGGTA2750.0144.06
CTAGGAG2150.0144.06
GATAGTG151.2614697E-4144.09
CTATAGA1100.0143.999986
CTATAAG1100.0143.999986
AGCTAGT15500.0143.999984
CTAGGAT1100.0143.999986
CAAGCTA114100.0143.810682
ACAAGCT114100.0143.810681
Produced by FastQC (version 0.11.5)