FastQCFastQC Report
Sat 18 Mar 2023
SRR4895595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113986
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA48474.252276595371361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA34923.063534118225045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25632.248521748284877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12561.1018897057533381No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA11611.018546137244925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10210.8957240362851578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6890.6044601968662818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5910.5184847261944449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA5620.4930430052813503No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAAAAAAAAAAAAAAAAAA4820.42285894759005493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACCTTTCATTTATTTGTGT3340.2930184408611584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCTGACGCCTATAATCC3250.28512273437088764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGAAAAAAAAAAAAAAA3050.2675767199480638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTCTTTATAGCTTATGACGA3010.264067517063499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA2740.24038039759268684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTGGCTCACACCTGTAAT2690.23599389398698084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAGAGAAAAAAAAAAAAAAAAAA2440.21406137595845104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAACAAAAAAAAAAAAAAA2280.20002456442019195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCCCTGAGAGATGAAAGTT2260.19826996297790958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAAAAAAAAAAAAAAAAA2150.18861965504535644No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACAAAAAAAAAAAAAAAA2080.1824785499973681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACTGACTTATTTTAAAAA2050.17984664783394452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGTGGTTGATATTGTATGA2040.17896934711280332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATTTATAGAATTTAAAGAC2010.17633744494937975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2010.17633744494937975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATTACCTGAGGTCAGGAG1990.17458284350709735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGCTGGCTGGGCGTGGTGG1980.17370554278595618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAATCCCAGCACTTTGGGAG1980.17370554278595618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAAAAAAAAAAAAAAAAAA1930.1693190391802502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATGCCCAGCTAATTTTTG1920.168441738459109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1900.16668713701682664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGATAAACAAAAATTAAC1880.16493253557454424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGGAGGATCCAGAGAA1880.16493253557454424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTGAAATCTTCAGAGA1860.16317793413226184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAGTGGCTCATGCCTG1840.16142333268997947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGGCTGGGCACCATGCCTC1840.16142333268997947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGATCATGTTAAAAACAT1840.16142333268997947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTTAACTTTTCTCCTGG1810.1587914305265559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAGGACAAGCGCGCGCGCA1810.1587914305265559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTAAAAAAAAAAAA1770.15528222764199112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTAACCAACATGGTGAAA1740.15265032547856755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTTTCGCTTGTAATCTCA1740.15265032547856755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGAAAAAAAAAAAAAA1720.15089572403628515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTTACAAAACAGGAGAGC1710.15001842331514398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGACTCCTGTGGTAAAAC1700.14914112259400278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCTAAGGCAGGTGGATC1680.14738652115172038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGTTTGAGGCTGAAATGC1680.14738652115172038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1670.1465092204305792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAACTCAGACACTTGTAAA1670.1465092204305792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1670.1465092204305792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTAGAAAGAGGGACAGAC1670.1465092204305792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGCTCATGCCTGTAA1670.1465092204305792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATTTATTGTTTAGCT1650.1447546189882968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGATCACTTGAGGCCAG1640.14387731826715563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGTTACAAAAAAAAAA1640.14387731826715563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAGGATGGAGTTCAGACA1600.14036811538259086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAGGAGTTCATGACCAGCC1600.14036811538259086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAACTGCTAAGGTCCACAA1590.13949081466144964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAACAAAAAAAAAAAAAA1580.13861351394030844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGCACGGTGGCTCAC1560.13685891249802606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAATACATGAAACAGAA1550.13598161177688486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA1540.1351043110557437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCCAAAATCAAGGCATTGGC1510.1324724088923201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTATAATTGGAAACAGAAA1500.13159510817117892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGCAGATCTTGCAGTGAG1490.13071780745003772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGACAGTGAGACTCCATCTC1490.13071780745003772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTCCCTGAGCAGGAGGAAT1480.12984050672889652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1470.12896320600775535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCCTGCTCTCTCACCAG1460.12808590528661415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAGAAATGCCTTCTAGTA1460.12808590528661415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAACCTAGGCATTACCATT1450.12720860456547295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGCAAGAGAAAAGCATAC1440.12633130384433175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1440.12633130384433175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAAGGCGGGCAGATCACC1430.12545400312319058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGTGGCTCATGCCTGT1430.12545400312319058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTTACTTCTAACTTAAAAAT1430.12545400312319058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCACTTTGGGGGGCCAAG1420.12457670240204938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCATCCCCGCGGAGCTGGT1390.1219448002386258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGAAATATAGGAAGGTATG1390.1219448002386258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAAAAAAAAAAAAAAAAA1390.1219448002386258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTGCATGGGGTAGATACC1390.1219448002386258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAACCGTATAATATCCTT1380.1210674995174846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA1370.12019019879634342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCAAGGCTGGCAGTTTG1360.11931289807520222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAACAGTCATATGACTGGT1350.11843559735406103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGGAGCTAAATAATGTGTA1340.11755829663291983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGTTACAAAAAAAAAAA1330.11668099591177865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGTATGTATGTTTTACA1330.11668099591177865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCACTTGAGGCCAAGGGT1330.11668099591177865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATTACCCGAGGTCAGGAG1320.11580369519063746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGGAGGCCAAGACAGGAGG1310.11492639446949626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACAACACCAAGCCAGTG1310.11492639446949626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGGATAAAAAAAAAAAA1290.11317179302721386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCCCCTGGGGTCTTTTCCC1290.11317179302721386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAAAAAATTTATAAAG1270.11141719158493148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCACATAGCCCGGTCGG1260.11053989086379028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCACGGGCGCAGATGTAGGC1260.11053989086379028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTACCAACGCAG1260.11053989086379028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGAGAGTCTGTTAACTCA1230.1079079887003667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA1190.10439878581580193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAAACCCCCAACCCAAAACC1190.10439878581580193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGATACCACAATCTATAGC1180.10352148509466075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTAAGGATTAACATTAAGAC1160.10176688365237836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATATCTGTTGTCTCCATTT1160.10176688365237836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCGGTGTGGTGGCTCACA1140.10001228221009598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT86000.0130.110261
TGGTATC87250.0128.31747
GTGGTAT87550.0127.877716
CAGTGGT87700.0127.741074
GGTATCA87650.0127.7318048
GTATCAA87850.0127.604939
AGCAGTG87750.0127.586242
AGTGGTA88450.0126.657925
GCAGTGG89050.0125.642833
TAAGCAG451.2070543E-464.251981
AACGTCG403.4539516E-728.79921245-49
CGGGTAG403.4539516E-728.799212140-144
GACAGCG403.4539516E-728.79921245-49
TCACCGT200.00628193128.79921280-84
AATCACG200.00628193128.799212105-109
GTCGACA403.4539516E-728.79921250-54
ACTGACG200.00628193128.79921290-94
CGTGCAC200.00628193128.799212105-109
GCTATCT200.00628193128.79921235-39
TAAACTC200.00628193128.799212120-124
Produced by FastQC (version 0.11.5)