FastQCFastQC Report
Sat 18 Mar 2023
SRR4895594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336812
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA1339900.0143.577292
GTCTGTC1340800.0143.480911
CTGTCAG390650.0143.265553
CTGTCAT438400.0143.237293
CTGTCAA338300.0142.782933
CTGTCAC178300.0141.973133
TGTCATG138500.0141.780154
TGTCATA126800.0141.58364
TGTCAGT129550.0141.521914
TGTCAGG131100.0141.111074
TGTCAGA95300.0140.965594
TGTCAAG109650.0140.825974
TGTCAAT116050.0140.562044
GTCATAG40950.0140.044515
TGTCACG49850.0139.579944
GTCATGG46750.0139.446725
GTCATGA37500.0139.113225
GTCATAA37250.0139.081025
GTCAGGT47200.0138.727025
TGTCATT128900.0138.662934
Produced by FastQC (version 0.11.5)