FastQCFastQC Report
Sat 18 Mar 2023
SRR4895594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336812
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57570.4306514304180393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37050.27715191066507483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21870.16359817236829113No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18800.14063308827269655No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC15960.11938851536341685No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA13510.10106133098745373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT275850.0138.899731
GGTATCA278400.0137.999948
GTGGTAT278500.0137.76956
GTATCAA280100.0137.136699
TGGTATC280600.0136.969287
AGTGGTA281350.0136.194855
AGCAGTG283050.0135.366522
CAGTGGT283650.0135.242754
GCAGTGG291300.0131.557483
CGTGGTA1453.669502E-529.781935
TCAACGC278950.028.21124610-14
ATCAACG278550.028.14323810-14
AGAGTAC280500.027.98351315-19
CAGAGTA281550.027.8791515-19
GTACATG281950.027.83907520-24
TACATGG282200.027.78380220-24
CAACGCA283900.027.75992610-14
GCAGAGT284150.027.6037915-19
AGTACAT283900.027.52615220-24
CGCAGAG286450.027.35215-19
Produced by FastQC (version 0.11.5)