FastQCFastQC Report
Sat 18 Mar 2023
SRR4895593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662872
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG1664350.0143.75541
TCGTAGA1664450.0143.746772
CGTAGAG468350.0143.531783
CGTAGAT538300.0143.457413
CGTAGAA446050.0143.433473
CGTAGAC216200.0142.620593
GTAGATA156400.0141.401144
GTAGATG170050.0141.388994
GTAGAGT140250.0141.21774
GTAGAAT153400.0141.071154
GTAGAGA127100.0139.922854
GTAGAAG140000.0139.876864
GTAGACG58950.0139.735724
GTAGACT52450.0139.495824
GTAGAGG152050.0139.390154
GTAGACC63000.0138.632114
GTAGAGC61100.0138.115544
GTAGATT152650.0137.993944
TAGATGG56800.0137.805425
TAGATGA45750.0137.123265
Produced by FastQC (version 0.11.5)