FastQCFastQC Report
Sat 18 Mar 2023
SRR4895593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662872
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97850.5884397596447593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20280.121957673230411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19890.1196123333605954No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18540.1114938491958491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263000.0138.018438
AAGCAGT263850.0137.648331
GTGGTAT265700.0136.48056
GTATCAA267050.0136.086999
TGGTATC267900.0135.547747
AGTGGTA269800.0134.433175
AGCAGTG271750.0133.461292
CAGTGGT272700.0133.109094
GCAGTGG276550.0131.196913
ATCAACG262550.028.22112310-14
TCAACGC264050.028.12621510-14
AGAGTAC264750.027.93869615-19
CAGAGTA265800.027.84457415-19
TACATGG266550.027.80548120-24
GTACATG268050.027.6337720-24
CAACGCA269300.027.62065310-14
GCAGAGT269050.027.45472715-19
AGTACAT269200.027.44619620-24
CGCAGAG271450.027.23319815-19
GAGTACA266450.027.18913820-24
Produced by FastQC (version 0.11.5)