FastQCFastQC Report
Sat 18 Mar 2023
SRR4895592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515830
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATC100.007231937144.040959
GTACGCA516200.0143.803761
TACGCAA516850.0143.622912
ACGCAAT175450.0143.464143
CGCAATA50150.0143.197864
ACGCAAG153050.0142.833923
CGCAATG57800.0142.794854
CGCAATT48000.0142.715994
CGCAAGT47100.0142.54034
ACGCAAC65900.0141.731433
GCAAGAT14750.0140.988195
CGCAAGA44100.0140.978234
GCAATGA15750.0140.717155
CGCAAAT37050.0140.225144
GCAATAG16900.0140.083225
CGCAAGG49650.0139.567384
CGCAATC20700.0139.39624
GCAATGT19300.0139.068395
ACGCAAA129400.0138.020773
GCAAGTA14450.0137.939565
Produced by FastQC (version 0.11.5)