FastQCFastQC Report
Sat 18 Mar 2023
SRR4895592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515830
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20670.40071341333385035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13930.2700502103406161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11450.2219723552333133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9670.1874648624546847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT8010.15528371750382877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC6230.12077622472520017No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC6100.1182560145784464No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA5580.1081751739914313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.0136.336278
AAGCAGT88150.0136.03761
GTGGTAT88200.0135.868236
GTATCAA88700.0135.264629
TGGTATC89000.0134.72787
AGTGGTA89600.0133.825625
CAGTGGT90400.0132.641314
AGCAGTG90900.0131.842822
GCAGTGG93300.0128.451353
TCAACGC88000.027.84063510-14
ATCAACG88350.027.6488810-14
AGAGTAC88150.027.59730315-19
CAGAGTA88550.027.5051515-19
TACATGG88550.027.46064420-24
CAACGCA89700.027.3611410-14
GTACATG89150.027.27582620-24
GCAGAGT89800.027.12228415-19
AGTACAT89600.027.09063520-24
GAGTACA88500.026.87425220-24
CGCAGAG90600.026.86690715-19
Produced by FastQC (version 0.11.5)