FastQCFastQC Report
Sat 18 Mar 2023
SRR4895591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719212
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA720000.0143.771592
TGCGAAT250300.0143.70153
GGTGCGA720400.0143.691761
TGCGAAG198800.0143.388523
GCGAATG80550.0142.85944
TGCGAAC88650.0142.7953
GCGAATA67900.0142.659674
TGCGAAA185100.0142.531863
GCGAATT71050.0142.513474
GCGAAGT62650.0142.093614
GCGAAAT61550.0141.709994
CGAAGTA19900.0141.400055
GCGAATC32950.0141.092214
CGAATGT29650.0141.018925
GCGAACT21250.0140.544894
CGAATTG23650.0140.279975
CGAATGA21600.0139.933435
GCGAAGA55800.0139.804444
GCGAAGG64800.0139.600254
CGAATAT23800.0139.395875
Produced by FastQC (version 0.11.5)