FastQCFastQC Report
Sat 18 Mar 2023
SRR4895590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230039
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAACA1238350.0142.919662
GGAGAAC1239300.0142.810091
AGAACAT385350.0142.396273
GAACAGT119000.0142.015694
AGAACAG380100.0141.353553
GAACATG122650.0141.13294
GAACATA108850.0140.386814
GAACAAT104000.0138.839894
GAACATT117200.0138.672034
AGAACAA315300.0137.409583
AGAACAC182850.0137.04363
GAACATC44050.0136.866874
AACAGTG40900.0136.67785
GAACAAG105750.0136.202154
GAACAGA104650.0135.365114
AACATAG36400.0135.193245
GAACACT47850.0133.816074
GAACAGG130200.0133.612054
AACATGA34550.0133.478165
GAACACG53000.0133.43754
Produced by FastQC (version 0.11.5)