FastQCFastQC Report
Sat 18 Mar 2023
SRR4895590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230039
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56680.4607983974491866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28800.2341389175465168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27580.22422053284489352No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13850.11259805583400202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13560.110240407011485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233200.0138.532068
AAGCAGT233600.0138.43791
GTGGTAT235550.0137.302726
GTATCAA235300.0137.265099
TGGTATC235750.0137.216777
CAGTGGT238700.0135.581254
AGTGGTA239150.0135.175675
AGCAGTG240450.0134.404242
GCAGTGG243850.0132.707373
TCAACGC234200.028.15965810-14
ATCAACG233600.028.15804710-14
AGAGTAC235300.027.94237715-19
TACATGG235550.027.9374120-24
CAACGCA236950.027.8571410-14
GTACATG236300.027.83046320-24
CAGAGTA236500.027.8249415-19
AGTACAT237450.027.64717120-24
CGCAGAG238550.027.53152315-19
GCAGAGT239050.027.49199915-19
AACGCAG240750.027.39950610-14
Produced by FastQC (version 0.11.5)