FastQCFastQC Report
Sat 18 Mar 2023
SRR4895589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886453
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA1887600.0143.827242
GCTCGGT1889450.0143.686431
TCGGTAT601750.0143.472473
TCGGTAA478700.0143.433913
TCGGTAG551250.0143.366683
CGGTATA168300.0143.05984
TCGGTAC262050.0142.953843
CGGTATG197150.0142.673974
CGGTAGT180100.0142.516544
CGGTAGA148800.0142.174014
CGGTAAG157850.0141.954734
CGGTAGG176150.0141.749884
CGGTAAT158150.0141.548934
CGGTATT181600.0141.180854
CGGTACG68250.0140.751954
CGGTATC60500.0140.346794
CGGTACT69050.0140.163334
CGGTAAA131700.0138.123354
CGGTAGC52450.0138.015644
CGGTACA61800.0137.976684
Produced by FastQC (version 0.11.5)