FastQCFastQC Report
Sat 18 Mar 2023
SRR4895589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886453
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85050.4508461117239603No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC57770.3062360949358399No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53490.283548013123041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATAGGGAGGAATGGAGAGT21920.11619690498517589No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21910.11614389544823008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19800.10495888315266799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT391950.0138.307021
GTGGTAT393350.0137.833166
GGTATCA393800.0137.712228
TGGTATC395450.0137.174017
GTATCAA396350.0136.9179
CAGTGGT399400.0135.817384
AGTGGTA400600.0135.302755
AGCAGTG402200.0134.692782
GCAGTGG405200.0133.73113
ATCAACG393400.028.09002110-14
TCAACGC395000.028.0746310-14
AGAGTAC396350.027.90274215-19
CAGAGTA397650.027.80066315-19
TACATGG397300.027.77987720-24
GTACATG397300.027.7726320-24
AGTACAT400000.027.54557420-24
CAACGCA403350.027.5255610-14
GCAGAGT402100.027.46435515-19
GAGTACA396000.027.3111820-24
CGCAGAG405750.027.21374915-19
Produced by FastQC (version 0.11.5)