FastQCFastQC Report
Sat 18 Mar 2023
SRR4895588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722.510460251046025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATGGCACCTGTCAAAAG331.1506276150627615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATGCATGGCTGAAAAGAG190.6624825662482566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCTGGGCGGGCCCAGCCA160.5578800557880056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA160.5578800557880056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAGACACATCTGGGACCA120.41841004184100417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG80.2789400278940028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG70.24407252440725244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA60.20920502092050208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCCGCTGCGTGTTTTCCT50.17433751743375175No Hit
CAAAGACAGGAAACGCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATC40.1394700139470014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA40.1394700139470014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA40.1394700139470014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCTTCCTAGATCCCCTC40.1394700139470014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAGCGAATTGGCACCAAA40.1394700139470014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.10460251046025104No Hit
GTATAGCTTCTGGCCTGGGCTTGGCCTGGATTGTTGGACGAGTTCTTTAT30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGACTCTATATAGCTGATCA30.10460251046025104No Hit
AATTTAGAAATATAATTGACAGGATTATTGGAAATTTGTTATAATGAATG30.10460251046025104No Hit
CTTCTTTATAATTCTACTTTGAGTGCTGTCTCCATGTTTGATGTATCTGA30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACAGTGGCTCACGCCT30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30.10460251046025104No Hit
ATTGACAGGATTATTGGAAATTTGTTATAATGAATGAAACATTTTGTCAT30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA30.10460251046025104No Hit
AGCATCAGATGAAGACTTCATTGGGTTTTATAGTGGCTTTCTGATTTTTG30.10460251046025104No Hit
CACAGGCTCGGGTCGCTGCAGACGCTTTCAGTGGGTTGACGGCAGCCGCT30.10460251046025104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA30.10460251046025104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1300.0138.461551
TGGTATC1350.0133.333337
AGTGGTA1350.0133.333335
GTATCAA1350.0133.333339
GTGGTAT1350.0133.333336
GGTATCA1350.0133.333338
AGCAGTG1400.0128.571432
GCAGTGG1400.0128.571433
CAGTGGT1400.0128.571434
ATGGGGC200.006080563628.825-29
ATGGGAT255.1092834E-428.825-29
GTACATG1350.028.820-24
TCAACGC1350.028.810-14
AGTACAT1350.028.820-24
CGCAGAG1350.028.815-19
CATGGGC200.006080563628.825-29
ATCAACG1350.028.810-14
GCAGAGT1400.027.7714315-19
AACGCAG1350.027.73333410-14
ACGCAGA1350.027.73333415-19
Produced by FastQC (version 0.11.5)