FastQCFastQC Report
Sat 18 Mar 2023
SRR4895587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910855
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC1914700.0143.594742
CCTCTAT1916300.0143.474851
TCTATCG555400.0142.951433
TCTATCA459200.0142.536713
CTATCGA138500.0142.117974
TCTATCC360750.0142.109183
CTATCAG144900.0141.997384
CTATCGT193850.0141.731554
TCTATCT556950.0141.533143
CTATCTA141400.0141.492774
CTATCAT156350.0141.491354
CTATCGG171850.0141.246674
CTATCTG183600.0141.023624
CTATCCG91850.0140.35944
TATCGTA56400.0139.419575
CTATCCA90100.0139.412634
CTATCAA123450.0139.280244
TATCGGA43150.0139.215745
TATCGAT50550.0138.760595
TATCGAG45550.0138.356125
Produced by FastQC (version 0.11.5)