FastQCFastQC Report
Sat 18 Mar 2023
SRR4895587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910855
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG126150.6601756805199767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74900.3919711333408344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC73410.38417357674967484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA36310.1900196508892616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT26040.13627407626429008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21740.11377106059852787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC20010.10471752173765148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT437950.0138.604061
GTGGTAT440100.0138.03086
GGTATCA440650.0137.923868
TGGTATC441550.0137.610147
GTATCAA444400.0136.857169
AGTGGTA446500.0135.971735
CAGTGGT448050.0135.661974
AGCAGTG451750.0134.322192
GCAGTGG456500.0133.019173
CGTGGTA1401.5108526E-841.1240125
TCAACGC441200.028.19632510-14
ATCAACG441100.028.1309310-14
CAACGCA446550.027.89074510-14
AGAGTAC444350.027.84743915-19
TACATGG445300.027.80325320-24
CAGAGTA445350.027.8010715-19
GTACATG446000.027.74670420-24
AGTACAT447950.027.57449720-24
CGCAGAG450350.027.48921215-19
GCAGAGT451350.027.40598515-19
Produced by FastQC (version 0.11.5)