FastQCFastQC Report
Sat 18 Mar 2023
SRR4895586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222372
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3280.1475005846059756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT100.007222595144.08499
CGTGGCA100.0072518135143.890498
CTGAGAT100.0072518135143.890498
CGATCTA100.0072518135143.890498
CTAATCC222750.0143.632112
TAATCCG78500.0143.432243
CCTAATC223250.0143.310421
TAATCCT68900.0142.637473
AATCCGA18500.0142.334924
AATCCTG22000.0142.255374
AATCCGT26250.0142.246034
ATCCTGA6100.0141.531635
ATCCGTG8450.0141.336215
TAATCCA39900.0141.185783
ATCCGAG5000.0141.012685
ATCCGTT9700.0140.923665
TCCGTGA2350.0140.8296
AATCCGG25650.0140.805114
TAATCCC37500.0140.628973
TCCGTAG2000.0140.293236
Produced by FastQC (version 0.11.5)