FastQCFastQC Report
Sat 18 Mar 2023
SRR4895586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222372
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9230.41507024265644954No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7110.3197344989477092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.25767632615617075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3460.15559512888313276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT2570.1155721044016333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2510.11287392297591424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC2300.10343028798589751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT2240.10073210656017845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT54300.0138.203741
GGTATCA54350.0137.983468
GTGGTAT54450.0137.465686
CAGTGGT54750.0136.84394
GTATCAA54900.0136.60119
AGTGGTA54800.0136.456385
TGGTATC55050.0135.967427
AGCAGTG55350.0135.321762
GCAGTGG56350.0132.79253
TCGCGGT200.006295909628.788486135-139
ATCAACG54250.028.31089210-14
TCAACGC54900.028.10679210-14
CAACGCA55000.028.05568910-14
AGAGTAC54750.027.9997615-19
GTACATG55250.027.85434220-24
CAGAGTA55100.027.79577815-19
TACATGG55550.027.60024620-24
AACGCAG55900.027.52673710-14
AGTACAT55650.027.52478420-24
CGCAGAG55850.027.49983615-19
Produced by FastQC (version 0.11.5)