FastQCFastQC Report
Sat 18 Mar 2023
SRR4895585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609987
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC16600.10310642259844334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC1615550.0143.414542
CCGACAA1617100.0143.277071
GACAACG483900.0143.1553
GACAACT462050.0143.0883
ACAACGT163500.0141.228764
GACAACA399350.0140.796193
ACAACTG151300.0139.870864
CAACGTA49450.0139.547965
GACAACC289450.0138.46913
CAACGGT55100.0138.428485
ACAACGG157500.0138.385284
ACAACTA128150.0137.961444
CAACGAT42000.0137.916995
ACAACGA126900.0137.56264
CAACGTG54050.0137.523155
CAACTAG43250.0137.42485
CAACGTT53500.0137.322975
CAACTAT44100.0137.223535
ACAACAT136000.0136.506074
CAACTGT54050.0136.058725
Produced by FastQC (version 0.11.5)