FastQCFastQC Report
Sat 18 Mar 2023
SRR4895585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609987
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85940.5337931300066397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG46560.2891948816978025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC42040.26112012084569625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20540.1275786698898811No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC19710.12242334875995893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC18000.11180214498626385No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC16490.10242318726797174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296800.0138.143748
AAGCAGT298400.0137.328021
GTGGTAT298900.0137.24546
TGGTATC299700.0136.95117
GTATCAA299500.0136.898369
AGTGGTA303100.0135.248645
CAGTGGT304550.0134.770134
AGCAGTG304400.0134.668472
GCAGTGG308750.0132.911093
CGTGGTA1102.5441378E-432.713445
TCAACGC298550.028.05499310-14
ATCAACG297850.028.01460810-14
AGAGTAC299000.027.89241815-19
CAGAGTA300250.027.7810915-19
TACATGG300650.027.74451320-24
GTACATG301100.027.67435620-24
CAACGCA303950.027.57550810-14
GCAGAGT303400.027.47842415-19
AGTACAT303050.027.44402920-24
GAGTACA299200.027.30637720-24
Produced by FastQC (version 0.11.5)