FastQCFastQC Report
Sat 18 Mar 2023
SRR4895584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438826
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5330.12146044217981615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCTC440650.0143.323962
ATCCTCG128450.0143.19993
CCATCCT441150.0143.161511
ATCCTCA110900.0142.435733
ATCCTCT128250.0142.07653
CCTCTAG11400.0141.403155
TCCTCGA30650.0141.11074
TCCTCAG35750.0140.908744
TCCTCGG41600.0139.949434
CCTCGTA14050.0139.31845
TCCTCTA36300.0139.170264
ATCCTCC77300.0138.994063
TCCTCAT39600.0138.658114
TCCTCGT45650.0137.780144
CCTCAGT13850.0137.693055
TCCTCTG39850.0137.246484
CCTCGAT11450.0137.014635
CCTCGGA10900.0136.665775
CTCGTAT5500.0136.077596
CCTCTGA9850.0135.89165
Produced by FastQC (version 0.11.5)