FastQCFastQC Report
Sat 18 Mar 2023
SRR4895583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636713
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC16930.10343902687887246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC1642100.0143.451642
CACCTTA1645600.0143.146531
CCTTACG451400.0142.936543
CTTACGT161500.0141.875244
CTTACGA113050.0141.633354
CCTTACT506000.0141.464553
CCTTACA416700.0141.351783
CTTACTA136100.0141.334084
CTTACTG152850.0141.147194
CTTACGG133850.0140.967944
CTTACAG125250.0140.477654
TTACGTA46450.0138.967365
CCTTACC292550.0138.63633
CTTACGC48750.0138.020324
TTACGGT49150.0137.629145
CTTACCG73300.0137.543554
TTACGAT43350.0137.28485
TTACGTG47600.0137.121775
TTACGGA35100.0136.339165
TTACTGA40550.0136.29395
Produced by FastQC (version 0.11.5)