FastQCFastQC Report
Sat 18 Mar 2023
SRR4895583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636713
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG85420.5218996855282508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73490.44900969198631646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC65620.4009255135139759No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA59240.36194494697604285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT25400.15518908935164566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCATGCCT17250.10539416501243651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT356150.0138.355791
GGTATCA358450.0137.446098
GTGGTAT360550.0136.685476
TGGTATC361700.0136.27087
GTATCAA361600.0136.208959
AGTGGTA365200.0134.905675
CAGTGGT367850.0134.051224
AGCAGTG369700.0133.32382
GCAGTGG377400.0130.565483
TCAACGC359450.028.129610-14
ATCAACG359300.028.09727310-14
AGAGTAC362500.027.82195515-19
CAGAGTA363350.027.7608315-19
GTACATG363800.027.69957520-24
TACATGG364050.027.64891220-24
CAACGCA366800.027.57378410-14
AGTACAT366500.027.479820-24
GCAGAGT368550.027.38867215-19
CGCAGAG370750.027.24168215-19
AACGCAG373800.027.06512310-14
Produced by FastQC (version 0.11.5)