FastQCFastQC Report
Sat 18 Mar 2023
SRR4895582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963236
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGAGC1973700.0143.159422
AGGAGCT566050.0143.124163
CAAGGAG1982250.0142.541931
GGAGCTA154700.0142.296984
AGGAGCG580050.0142.175813
AGGAGCA482100.0142.029393
GGAGCAT161400.0141.428274
GGAGCGT196200.0141.064214
GAGCTAG52700.0140.101655
AGGAGCC361150.0140.099323
GGAGCTG178150.0139.724094
GGAGCTT174800.0139.190724
GGAGCGA147650.0138.85634
GAGCTAA43450.0138.790835
GAGCTAT54000.0138.72785
GAGCATA46900.0138.247885
GAGCGTA54350.0137.966835
GAGCTGA46150.0137.843755
GAGCAAT47350.0137.389955
GAGCGAT53950.0137.389115
Produced by FastQC (version 0.11.5)