FastQCFastQC Report
Sat 18 Mar 2023
SRR4895581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087622
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG93010.8551684316793886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44210.40648313476557113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.3754061613317862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22810.20972359882385608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA15820.14545494666345476No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA12620.1160329599805815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT229750.0137.473131
GTGGTAT231250.0136.8056
GGTATCA231700.0136.477178
TGGTATC232450.0136.06787
GTATCAA232850.0135.710429
AGTGGTA235450.0134.456335
CAGTGGT235600.0134.309634
AGCAGTG236350.0133.634232
GCAGTGG244550.0129.241683
TCAACGC231600.027.92247210-14
ATCAACG231550.027.89120510-14
AGAGTAC232950.027.62472315-19
CAACGCA234950.027.5733510-14
CAGAGTA233650.027.52348315-19
TACATGG233950.027.5006620-24
GTACATG234100.027.4891920-24
AGTACAT234550.027.33824320-24
GCAGAGT236450.027.19146715-19
CGCAGAG237300.027.11832815-19
AACGCAG239800.027.03368210-14
Produced by FastQC (version 0.11.5)