FastQCFastQC Report
Sat 18 Mar 2023
SRR4895580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176874
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC16420.13952215785207253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC1180200.0143.50171
TCATTCC1182600.0143.210462
CATTCCG397450.0143.023093
CATTCCT335300.0141.65693
ATTCCGA98100.0141.489674
ATTCCGT140950.0141.357684
CATTCCA253850.0141.018943
ATTCCTG105050.0139.526444
ATTCCTA101050.0139.13934
ATTCCGG121350.0138.988654
ATTCCGC44850.0137.653214
TTCCGAT42450.0137.468965
ATTCCAG75550.0137.433754
TCCGATG14750.0136.104896
TTCCGAG26400.0136.006035
CATTCCC214900.0135.97473
ATTCCAT98300.0135.492254
TTCCGTA38650.0135.160145
TCCGATA11700.0134.070166
TTCCGTG46000.0133.899055
Produced by FastQC (version 0.11.5)