FastQCFastQC Report
Sat 18 Mar 2023
SRR4895580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176874
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG46610.39604919473112665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30300.2574617163774542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29500.2506640472981814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC21090.17920355110232702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG16790.14266607980123616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA14950.12703144091890892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC12180.10349451173192713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12130.10306965741447256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT281050.0139.248031
GTGGTAT283700.0137.70726
GGTATCA283800.0137.658668
TGGTATC285100.0137.106727
GTATCAA285650.0136.716759
AGTGGTA286750.0136.24255
AGCAGTG288200.0135.693442
CAGTGGT288800.0135.499684
GCAGTGG294000.0133.01653
TCAACGC283500.028.29203410-14
ATCAACG284100.028.13601310-14
AGAGTAC284850.028.06698615-19
CAGAGTA286500.027.92041615-19
GTACATG286700.027.88057320-24
CAACGCA288300.027.86592910-14
TACATGG287150.027.84690920-24
AGTACAT287350.027.72732220-24
GCAGAGT290050.027.58365415-19
CGCAGAG289950.027.5733115-19
AACGCAG292450.027.46065310-14
Produced by FastQC (version 0.11.5)