FastQCFastQC Report
Sat 18 Mar 2023
SRR4895579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614333
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA2616650.0143.784772
GCTAACG2616900.0143.771041
TAACGAT823350.0143.564443
TAACGAG798450.0143.535523
TAACGAC358450.0143.030753
TAACGAA645750.0142.710573
AACGATG270000.0142.528184
AACGAGT234600.0142.165714
AACGATA236850.0142.091174
AACGAAT217700.0141.46814
ACGATAG79450.0140.653555
AACGATT244750.0140.41544
ACGATGA73700.0140.301545
ACGAGTA70600.0140.244845
AACGATC83650.0139.871024
ACGAGGT88550.0139.76975
AACGAGG280450.0139.423924
AACGACT92300.0139.158484
ACGATTG87500.0139.062075
ACGAATG74900.0139.01445
Produced by FastQC (version 0.11.5)