FastQCFastQC Report
Sat 18 Mar 2023
SRR4895579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614333
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114760.4389647378509165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC53790.2057503768647682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50840.19446642795695882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT30970.11846233819486653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG28070.10736964265837597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC28050.10729314130984843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCTTCCTAGATCCCCTC26580.10167029219307563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT460550.0138.05381
GGTATCA461500.0137.690788
GTGGTAT463050.0137.245446
TGGTATC466500.0136.292147
GTATCAA466400.0136.228789
AGCAGTG471200.0134.94882
CAGTGGT472300.0134.648914
AGTGGTA472900.0134.356325
GCAGTGG483050.0131.593573
TCAACGC460450.028.16053810-14
ATCAACG460750.028.07972110-14
AGAGTAC466050.027.81971715-19
TACATGG465600.027.81183820-24
GTACATG466700.027.71234920-24
CAGAGTA467900.027.70664615-19
CAACGCA472350.027.51203310-14
AGTACAT470350.027.4758720-24
GCAGAGT472850.027.3678915-19
GAGTACA464900.027.25290920-24
CGCAGAG476600.027.15557315-19
Produced by FastQC (version 0.11.5)