FastQCFastQC Report
Sat 18 Mar 2023
SRR4895578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538228
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3541900.0143.750151
TAGCGAC3542250.0143.735952
AGCGACT1014550.0143.43243
AGCGACG1004900.0143.170153
GCGACTA275700.0143.039264
GCGACAT310650.0142.835054
GCGACGT338600.0142.816744
AGCGACA929650.0142.801473
GCGACTG310550.0142.649374
GCGACTT325600.0142.154754
AGCGACC612350.0142.079253
CGACGTA101300.0141.556585
GCGACGA263450.0141.497124
CGACATG101950.0141.006945
CGACTGA84000.0140.731235
GCGACGG316100.0140.463424
CGACGAT92550.0140.324345
CGACTAT101050.0140.055535
CGACTAG87550.0140.037925
GCGACAA263250.0139.910064
Produced by FastQC (version 0.11.5)