FastQCFastQC Report
Sat 18 Mar 2023
SRR4895578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538228
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG121220.34260087252715204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119270.33708963922053636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC68710.19419325153721015No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT65080.18393387876643338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCTTCCTAGATCCCCTCC41500.11729034985874286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT40790.11528369568043664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT38030.10748318084645761No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC36830.10409165265777107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT665450.0138.561191
GGTATCA666350.0138.342858
GTGGTAT669250.0137.732626
TGGTATC670100.0137.600867
GTATCAA673250.0136.91439
AGTGGTA677850.0135.94275
CAGTGGT679600.0135.762084
AGCAGTG682750.0135.050222
GCAGTGG695650.0132.597633
TCAACGC666800.028.24567810-14
ATCAACG666100.028.2018910-14
AGAGTAC670100.028.00394815-19
CAGAGTA672200.027.9121815-19
TACATGG672600.027.84163920-24
GTACATG677700.027.6576120-24
CAACGCA683250.027.5972310-14
GCAGAGT683400.027.47790515-19
AGTACAT681900.027.4513720-24
GAGTACA670100.027.39764220-24
CGCAGAG689050.027.24841715-19
Produced by FastQC (version 0.11.5)