FastQCFastQC Report
Sat 18 Mar 2023
SRR4895577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697127
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC2700100.0143.770972
AGGCTAA2702100.0143.664571
GCTAACT813800.0143.64793
GCTAACG759850.0143.618353
GCTAACA676250.0143.079543
GCTAACC457700.0142.908873
CTAACTG260950.0142.496814
CTAACGT253700.0142.399114
CTAACGG240600.0141.837134
CTAACGA197550.0141.745154
CTAACTA218400.0141.69024
CTAACAT232650.0140.497334
CTAACTT258750.0140.148364
CTAACCG119250.0139.585824
CTAACAG214050.0139.358464
TAACGGT80450.0139.189835
TAACGTG77300.0139.089755
TAACTAG73350.0138.730945
TAACGAT69600.0138.554155
TAACTGG85750.0137.804385
Produced by FastQC (version 0.11.5)