FastQCFastQC Report
Sat 18 Mar 2023
SRR4895577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697127
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117820.43683519537641347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC62340.23113483347280273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47750.1770402357768099No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCTTCCTAGATCCCCTC38660.14333770712317218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC33180.12301979105915294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG31920.11834815342399523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCTTCCTAGATCCCCTCC29180.10818919539198563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT413100.0138.602431
GGTATCA412450.0138.531658
GTGGTAT416950.0137.088336
GTATCAA417300.0137.025099
TGGTATC417500.0136.97677
AGCAGTG423200.0135.243532
CAGTGGT423250.0135.217864
AGTGGTA425650.0134.23565
GCAGTGG440200.0129.987853
TCAACGC413350.028.26941510-14
ATCAACG412900.028.20260410-14
AGAGTAC414600.028.09074815-19
CAGAGTA416050.027.97900815-19
GTACATG416100.027.95505320-24
TACATGG416850.027.92893420-24
AGTACAT419950.027.66791220-24
GAGTACA414350.027.48587820-24
GCAGAGT424000.027.47137615-19
CAACGCA428550.027.2835410-14
ACATGGG429300.027.07537720-24
Produced by FastQC (version 0.11.5)