FastQCFastQC Report
Sat 18 Mar 2023
SRR4895576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883384
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG1885800.0143.726851
GCCATGC1887350.0143.608812
CCATGCG546600.0143.163473
CCATGCT506650.0142.948043
CCATGCA439000.0142.782843
CATGCGT179650.0142.191514
CATGCGG177000.0141.92184
CATGCGA135200.0141.891374
CATGCTG158700.0141.601414
CATGCAG134100.0141.17724
CATGCTA137200.0140.976824
CCATGCC409650.0140.804733
CATGCAT148250.0140.710344
CATGCCG106950.0140.48254
CATGCTT157500.0140.258884
ATGCGTA52700.0139.817615
ATGCGGT62250.0138.94335
CATGCGC60500.0138.918474
ATGCATA41750.0138.570925
ATGCGTG54850.0138.141535
Produced by FastQC (version 0.11.5)