FastQCFastQC Report
Sat 18 Mar 2023
SRR4895576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883384
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75260.39959986917166124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG48200.25592231855001424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38740.20569358134082055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC31340.16640260297422088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG29760.1580134481337847No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT23360.12403206143834715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20560.1091652047590932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT349050.0138.117391
GGTATCA350450.0137.535038
GTGGTAT351400.0137.18376
GTATCAA353750.0136.231679
TGGTATC354100.0136.198647
CAGTGGT356150.0135.475334
AGCAGTG358000.0134.724782
AGTGGTA358750.0134.413225
GCAGTGG367850.0131.156373
ATCAACG350150.028.03210410-14
TCAACGC351400.028.0061210-14
AGAGTAC351450.027.91657315-19
TACATGG353650.027.76071720-24
CAGAGTA353950.027.73972515-19
GTACATG355650.027.56007620-24
AGTACAT357850.027.35845620-24
GAGTACA352050.027.29798520-24
GCAGAGT359450.027.29124615-19
CAACGCA364800.027.02473410-14
ACATGGG361800.026.96028120-24
Produced by FastQC (version 0.11.5)