FastQCFastQC Report
Sat 18 Mar 2023
SRR4895575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792223
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1796200.0143.604191
GCACCTC1797450.0143.504322
CACCTCG522500.0142.574973
ACCTCGA132000.0141.853064
ACCTCGT171550.0141.617554
CACCTCT534300.0141.365683
CACCTCA441500.0141.284183
ACCTCAG133650.0140.801764
ACCTCTG166050.0140.3714
ACCTCTA150650.0139.959984
ACCTCAT149750.0139.791984
CCTCGTA47900.0139.718125
ACCTCGG168250.0139.390984
CCTCGAT47900.0137.614855
CCTCGAG40650.0136.66655
CCTCAGT45350.0136.149285
CACCTCC334750.0136.035173
CCTCTAG48500.0135.467275
CCTCTGA43750.0135.371465
ACCTCCG86000.0135.305954
Produced by FastQC (version 0.11.5)