FastQCFastQC Report
Sat 18 Mar 2023
SRR4895575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792223
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79320.44257885318958634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG66860.37305625471830234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61950.3456601103768895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA31250.17436446245807583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC26340.14696831811666292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG23330.13017353309270108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC18930.105623016778604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA335900.0137.997748
GTGGTAT336650.0137.604776
AAGCAGT337100.0137.370181
GTATCAA337400.0137.170889
TGGTATC338300.0136.976187
CAGTGGT343050.0135.058584
AGTGGTA344600.0134.430195
AGCAGTG344850.0134.282972
GCAGTGG351800.0131.65063
TCGTACG304.624673E-528.79143550-54
ATCAACG334450.028.13240810-14
TCAACGC335750.028.0792210-14
AGAGTAC336600.027.99261315-19
TACATGG335850.027.96503320-24
CAGAGTA338100.027.830115-19
GTACATG338100.027.80448320-24
AGTACAT339000.027.67545120-24
GAGTACA336800.027.38597320-24
CAACGCA345300.027.36933510-14
GCAGAGT344550.027.3049415-19
Produced by FastQC (version 0.11.5)