FastQCFastQC Report
Sat 18 Mar 2023
SRR4895574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123416
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA39283.1827315745122187No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA36602.9655798275750307No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA29662.403254035133208No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTTGATA25112.0345822259674597No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA20881.6918389836001815No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA19011.5403189213716213No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACACTGCGTTGATAC14001.1343747974330718No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGCGGATA8970.7268101380696181No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC7860.6368704219874246No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACTCTGCGTTGATA6670.5404485642056135No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACGCTGCGTTGATA6600.5347766902184482No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACTCTGCGTTGATAC6480.5250534776690219No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGGACTCTGCGTTGATA6360.5153302651195955No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGGACGCTGCGTTGATA6150.49831464315809937No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACCCTGCGTTGATA5830.4723860763596292No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTGGATA5200.42133921047514095No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGGACTCTGCGTTGATA4900.3970311791015752No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGGACGCTGCGTTGATA4830.39135930511440986No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCACGGACTCTGCGTTGATA4700.3808258248525313No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGGACGCGGCGTTGATA4660.3775847540027225No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACGCTGCGTTGATA4570.37029234459065274No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCGTTGATA4490.36381020289103516No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGGACGCTGCGTTGATA4450.3605691320412264No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATA4430.35894859661632206No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACTCTGCGTTGATA4360.35327672262915666No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCACGGACGCTGCGTTGATA4280.34679458092953913No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGTACTCTGCGTTGATA4250.3443637777921825No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGTACTCTGCGTTGATA3640.2949374473325987No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACGCGGCGTTGATA3520.28521423478317237No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACGCGGCGTTGATA3500.28359369935826795No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGGGTA3500.28359369935826795No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGCTGATA3280.2657678096843197No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGTTGATA3230.2617164711220587No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGGACGCGGCGTTGATA3210.26009593569715433No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGGACTCTGCGTTGATA3210.26009593569715433No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGGACGCGGCGTTGATA3170.25685486484734554No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCATGTACTCTGCGTTGATA3090.250372723147728No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATA3040.24632138458546704No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACACAGCGTTGATAC2910.2357879043235885No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACACTGCGTTGATAC2760.22363388863680558No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCACGGACGCGGCGTTGATA2660.21553121151228366No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGTACTCTGCGTTGATA2630.21310040837492708No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCACGTACTCTGCGTTGATA2610.21147987295002268No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCATGGACACTGCGTTGATAC2570.2082388021002139No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATA2420.196084786413431No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATA2420.196084786413431No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGGACGCGGCGCGGATA2350.19041291242626565No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACCCTGCGTTGATA2320.18798210928890904No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCACGGACCCTGCGTTGATA2310.18717184157645686No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACCCTGCGTTGATAC2270.1839307707266481No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCATGGACACTGCGTTGATAC2240.18149996758929152No Hit
AGATGTACTCTGCGTTGATACCACCGCCTCCCACGGACTCTGCGTTGATA2230.1806896998768393No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCATGGACACTGCGTTGATAC2220.17987943216438712No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCATGGACTCTGCGTTGATA2200.17825889673948273No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATA2130.17258702275231738No Hit
AGATGTACTCTGCGTTGATACCACCGCCTCCCACGGACGCTGCGTTGATA2120.17177675503986517No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGGACTCTGCGTTGATA2090.1693459519025086No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCATGTACTCTGCGTTGATA2080.16853568419005638No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCATGGACACTGCGTTGATAC2060.166915148765152No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATA2020.16367407791534325No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGTACTCTGCGTTGATA1990.16124327477798664No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGGACTCTGCGTTGATA1990.16124327477798664No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCGGCGTTGATA1990.16124327477798664No Hit
AGATGTACTCTGCGTTGATACCACCGCTCCCCACGGACTCTGCGTTGATA1900.1539508653659169No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACGCGGCGCGGATA1860.1507097945161081No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCATGGACACTGCGTTGATAC1850.14989952680365592No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCATGGACTCTGCGTTGATA1800.14584818824139495No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACCCTGCGTTGATA1790.14503792052894277No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGGACTCTGCGTTGATA1740.1409865819666818No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGGACTCTGCGTTGATA1740.1409865819666818No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACGCGGCGCGGATA1720.13936604654177742No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACCCTGCGTTGATA1680.13612497569196863No Hit
AGATGTACTCTGCGTTGATACCACCGCTCCCCACGGACGCTGCGTTGATA1670.13531470797951642No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCCTGCGTTGATA1600.12964283399235108No Hit
AGATGTACTCTGCGTTGATACCACTGCCTCCCACGGACCCTGCGTTGATA1450.11748881830556815No Hit
AGATGTACTCTGCGTTGATACCACCGCCTCCCACGTACTCTGCGTTGATA1430.11586828288066378No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCATGTACTCTGCGTTGATAC1390.112627212030855No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACACAGCGTCGAGAC1380.11181694431840279No Hit
AGATGTACTCTGCGTTGATACCACGGCTTCCCACGGACTCTGCGTTGATA1360.11019640889349841No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGGACGCGGCGCGGATA1320.10695533804368965No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACCCTGCGTTGATAC1320.10695533804368965No Hit
AGATGTACTCTGCGTTGATACCACAGCTCCCCACGGACTCTGCGTTGATA1310.10614507033123745No Hit
AGATGTACTCTGCGTTGATACCACAGCTCCCCACGGACGCTGCGTTGATA1290.10452453490633305No Hit
AGATGTACTCTGCGTTGATACCACCGCCTCCCACGGACGCGGCGTTGATA1280.10371426719388087No Hit
AGATGTACTCTGCGTTGATACCACGGCTTCCCACGGACGCTGCGTTGATA1280.10371426719388087No Hit
AGATGTACTCTGCGTTGATACCACTGCTCAGAACGGAAGAGCGGCGGGTA1270.10290399948142866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG100.007234445143.980139
TAATGCG100.007234445143.980139
GTACCTT253.8602593E-8143.92185
GTACCGA306.8030204E-10143.92185
TACTTGA253.8602593E-8143.92186
GTACAGT700.0143.92185
GTACAGG650.0143.92185
TACTTAG253.8602593E-8143.92186
GTACAAT700.0143.92185
TACGGTC253.8602593E-8143.92186
ACTTAGT151.2644543E-4143.92187
ACGATGT151.2644543E-4143.92187
TACGAGA151.2644543E-4143.92186
TACTGAG306.8030204E-10143.92186
GTACTAC253.8602593E-8143.92185
TACCTGC151.2644543E-4143.92186
TACCTGA151.2644543E-4143.92186
TACTAAT306.8030204E-10143.92186
TACAGTG253.8602593E-8143.92186
TACAGTA306.8030204E-10143.92186
Produced by FastQC (version 0.11.5)