FastQCFastQC Report
Sat 18 Mar 2023
SRR4895573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916695
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1920900.0143.594832
AGATTCG561850.0143.593053
ACAGATT1921700.0143.535051
AGATTCA464400.0142.999053
AGATTCC342850.0142.27623
GATTCGA145850.0142.235814
AGATTCT562600.0142.13543
GATTCGG170350.0142.012424
GATTCGT196650.0141.607994
GATTCTG175850.0140.967044
GATTCAT159700.0140.89444
GATTCAG143650.0140.857644
GATTCCG84750.0140.601964
GATTCAA129000.0140.00864
GATTCGC56500.0139.710454
ATTCGTA57850.0139.43545
GATTCTA155800.0138.832864
ATTCGAG45500.0138.69445
ATTCGGA42100.0138.614765
ATTCGAT55900.0138.249395
Produced by FastQC (version 0.11.5)