FastQCFastQC Report
Sat 18 Mar 2023
SRR4895573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916695
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82190.4288110523583564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG77960.40674181338188914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC47070.24557897839771062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT40300.2102577614070053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22960.11978953354602584No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC22930.11963301412066082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC22040.11498960450149869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG21250.1108679263002199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19900.10382455215879419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT401600.0138.180391
GGTATCA401850.0138.076228
GTGGTAT403400.0137.706276
TGGTATC404950.0137.179177
GTATCAA405900.0136.680799
AGTGGTA409350.0135.75745
CAGTGGT410800.0135.470954
AGCAGTG415050.0133.823992
GCAGTGG426950.0130.245823
TCAACGC403050.028.14732710-14
ATCAACG403650.028.02346810-14
TACATGG405650.027.86851520-24
AGAGTAC406950.027.8245215-19
CAGAGTA407850.027.77723915-19
GTACATG408800.027.70660620-24
CAACGCA410400.027.67128810-14
GCAGAGT410350.027.60099415-19
AGTACAT409950.027.56566420-24
CGCAGAG413500.027.34895515-19
GAGTACA405950.027.27691720-24
Produced by FastQC (version 0.11.5)