FastQCFastQC Report
Sat 18 Mar 2023
SRR4895572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388380
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5280.13594932797775375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC389700.0143.419941
CACGACC390050.0143.291242
ACGACCG131900.0143.209273
ACGACCT108300.0142.988283
CGACCGT41400.0142.354174
GACCGTG13200.0142.283075
ACGACCA78150.0142.26113
GACCGTA11350.0141.38255
CGACCAT26850.0141.238454
GACCGAT11850.0140.882255
GACCTAG8900.0140.684375
GACCATG8750.0140.628945
CGACCTG36600.0140.576134
CGACCGA31700.0140.286484
CGACCTT31750.0140.065574
CGACCTA31100.0139.985054
GACCTAT11400.0139.499955
ACGACCC74700.0139.198273
CGACCGG44300.0138.395684
ACCGATA3800.0138.23756
Produced by FastQC (version 0.11.5)